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2 edition of Studies on the hemolytic toxins from sea anemone nematocyst venom. found in the catalog.

Studies on the hemolytic toxins from sea anemone nematocyst venom.

Gary R. Grotendorst

Studies on the hemolytic toxins from sea anemone nematocyst venom.

by Gary R. Grotendorst

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Published by University of South Florida in [Tampa, Florida] .
Written in English


ID Numbers
Open LibraryOL13722075M

  The nematocyst is one of the most complex intracellular structures found in nature and is the defining feature of the phylum Cnidaria (sea anemones, corals, jellyfish, and hydroids). This miniature stinging organelle contains and delivers venom into prey and foe yet little is known about its toxic components. In the present study, we identified by tandem mass spectrometry 20 proteins released. Venom from the sea anemone, Heteractis magnifica, has multiple biological effects including, cytotoxic, cytolytic and hemolytic activities. In this study, cytotoxicity induced by H. magnifica venom was investigated using the crystal violet assay on human breast cancer T47D and MCF7 cell lines and normal human breast B5 cell line. Apoptosis was also assayed via Annexin V-flourescein.

ABSTRACT. The unifying characteristic of cnidarians is the production of protein and polypeptide toxins. The present study describes the identification of a hemolytic toxin from the soft coral Sarcophyton crude extract was highly cytotoxic (EC 50 = 50 ng/mL) against human erythrocytes. It was also tested for hemolytic activity by the blood agar plate method, resulting in a. A pharmacological study of the toxin of a Cnidarian, Chironex Drago Lebez, Isolation and characterization of three lethal and hemolytic toxins from the sea anemone Actinia equina L., Toxicon Allen P. Kaplan, Mediators present in the nematocyst venoms of the sea nettle, sea wasp and Portuguese man-o'war, Comparative Biochemistry.

  Chinese researchers recently reported on venomous toxins of the giant jellyfish. They revealed that this aquatic creature mixes a “cocktail” of toxins to produce stinging agony and sometimes death unto its victims.1,2 Jellyfish stings are a major threat to human beings in coastal areas of the world. Each year, hundreds of thousands of victims are stung by venomous jellyfish. Hemolytic, phospholipase a and nerve-affecting activities of sea anemone nematocyst venom. Nat New Biol. ; – [Google Scholar] Honma T, Nagai H, Nagashima Y, Shiomi K. Molecular cloning of an epidermal growth factor-like toxin and two sodium channel toxins from the sea anemone Stichodactyla gigantea. Biochim Biophys Acta.


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Studies on the hemolytic toxins from sea anemone nematocyst venom by Gary R. Grotendorst Download PDF EPUB FB2

Nagai H, Oshiro N, Takuwa-Kuroda K, Iwanaga S, Nozaki M, Nakajima TNovel proteinaceous toxins from the nematocyst venom of the Okinawan sea anemone Phyllodiscus semoni Kwiet-niewski Biochem Biophys Res Commun, (), pp.

Cited by: 5. Several hemolytic protein toxins have been isolated from P. semoni venom, including a kd molecular weight protein, PsTXA, and kd molecular weight proteins 60A and 60B, 3 whereas other sea anemone venoms contain hemolytic, neurotoxic, and cardiotoxic protein toxins.

4, 5, 6 However, there have been no previous reports describing Cited by:   A study of the nematocyst venoms of the sea anemone, Bolocera tuediae. ToxiNematocyst venoms from the mesen- teric filament and tentacles of Bolocera tuediae contain toxins capable of killing mice and hemolyzing human by: Hessinger DA, Lenhoff HM, Kahan LB.

Hemolytic, phospholipase a and nerve-affecting activities of sea anemone nematocyst venom. Nat New Biol. ; – Honma T, Nagai H, Nagashima Y, Shiomi K. Molecular cloning of an epidermal growth factor-like toxin and two sodium channel toxins from the sea anemone Stichodactyla by:   A recent study on CfTX toxins showed that CfTX-1/2 caused cardiovascular collapse within 1 min in anesthetized rats exposed in vivo to the venom, whereas CfTX-A/B more potent in eliciting in vitro hemolytic activity.

Although these data suggest that the jellyfish toxin family has undergone functional diversification, more structural/functional Cited by:   The few toxins which have been purified sufficiently for such studies were found to interact with specific membrane lipids: (1) basic protein cytotoxins from the sea anemone Stoichactis and the nemertine Cerebratulus were inhibited by small amounts of sphingolipids, (2) phospholipase A's and a protein colytic factor from the nematocyst venom of.

Purchase Toxins - 1st Edition. Print Book & E-Book. ISBNText node here in '{0}'. Pharmacological Studies on the Venom of a Sea Snake, Hydrophis Cyanocinctus The Mechanism of Sea Anemone Nematocyst Venom - Induced Hemolysis.

Biologically active compounds from the Brazilian sea anemone Bunodosoma caissarum have been studied chemically and pharmacologically for many years. The purpose of this work was to continue these studies focusing on the toxins contained in the nematocysts, as well as their activities, because many toxic effects were attributed to sea anemones.

This suggests an extremely high toxicity of A. mutabilis venom, since previous studies on toxicity of jellyfish and sea-anemone venoms, namely from P.

noctiluca (Mariottini et al., ) and from A. aequorea, R. pulmo and A. sulcata (Carli et al., ) have reported IC 50 values betw nematocysts per mL, with even longer.

In the present study, we isolated a novel hemolytic toxin from globular vesicles of tentacles and found that it has biochemically and physiologically similar properties to those of hemolysins from other species of sea anemone (Nagai et al.,Macek and Lebez,Kem and Dunn,Simpson et al.,Wang et al., ).

The full-length cDNA that encodes the hemolytic toxin Avt-I, with amino acids, from the venomous sea anemone Actineria villosa has been cloned using the oligo-capping method.

Venoms from jellyfish; CORALS; SEA ANEMONES; etc. They contain hemo- cardio- dermo- and neuro-toxic substances and probably ENZYMES. They | Explore the latest full-text research PDFs. The actinoporin family is one of the four most common toxin protein families that are isolated from sea anemones together with sea anemone sodium channel inhibitory toxin family, type I subfamily, sea anemone type 3 (BDS) potassium channel toxin family, and venom Kunitz-type family, sea anemone type 2 potassium channel toxin subfamily [].The actinoporins have mainly been found in the.

Nagai H, Oshiro N, Takuwa-Kuroda K, Iwanaga S, Nozaki M, Nakajima TA. New polypeptide toxin from the nematocyst venom of an Okinawan sea anemone Phyllodiscus semoni (Japanese name “unbachi-isoginchaku”). Biosci Biotechnol Biochem. b;66(12)–5. CrossRef PubMed Google Scholar.

Abstract. Biochemical and biological properties of 30 cytolytic polypeptide toxins isolated from 18 species of sea anemones (Actiniaria) are presented and classified into three groups according to their molecular mass, isoelectric points and the molecular mechanism of olipase A 2-like toxins (30 kDa) from Aiptasia pallida are dissimilar to acidic metridiolysin (80 kDa) from.

Intriguingly, the nematocyst-derived fractions were often less toxic than whole tentacle extracts, raising the possibility that toxins may also reside in non-nematocyst compartments [11,12]. Among the better studied cnidarian venom components are the potent sea anemone Type I neurotoxins.

Objective: In vitro hemolytic activity of the tentacle extract of Paracondylactis indicus (Dave), a sea anemone found in the eastern coastal region of West Bengal (India), was determined in rat erythrocytes.

Materials and Methods: Acute toxicity study was carried out with the tentacle extract followed by detailed biochemical studies to evaluate the direct and indirect hemolytic activities of. A kDa protein toxin (bandaporin) was isolated from the sea anemone Anthopleura asiatica (Actiniidae) using the precipitation of ammonium sulfate saturation, and ion-exchange and gel-permeation chromatographies.

Bandaporin showed potent hemolytic activity (EC50 value: ng/ml to % suspension of sheep red blood cells) and lethal toxicity to crayfish (LD value: mg/kg). Hessinger DA, Lenhoff HM. Degradation of red cell membrane phospholipids by sea anemone nematocyst venom.

Toxicon. Aug; 12 (4)– Norton TR, Kashiwagi M. Purification of a potent antitumor agent from a Tahitian sea anemone and methods of administration.

Studies with Ehrlich ascites tumor in mice. This means all the cnidarians have venom. So far, a number of polypeptide toxins have been isolated and characterized from the sea anemones. Among these studies, ion-channel toxins and pore forming toxins have been characterized.

Some ion channel toxins are revealed to be useful tools for biomedical and physiological science. A double-walled capsule found in jellyfish and other CNIDARIA whose functions include prey capture, defense, locomotion, and attachment.

| Explore the latest full-text research PDFs, articles.Properties of a toxin from the sea anemone Stoichacis helianthus, including specific binding to sphingomyelin. Proc Natl Acad Sci U S A. Feb; 73 (2)– [PMC free article] [Google Scholar] Burnett JW, Calton GJ.

A comparison of the toxicology of the nematocyst venom from sea nettle fishing and mesenteric tentacles. Toxicon.Abstract. Sea anemones are a rich source of biologically active substances. In crayfish muscle fibers, Bunodosoma cangicum whole venom selectively blocks the I K(Ca) currents.

In the present study, we report for the first time powerful hemolytic and neuroactive effects present in two different fractions obtained by gel-filtration chromatography from whole venom of B.

cangicum.